megascript t7 transcription kit am1334 (Thermo Fisher)

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Thermo Fisher megascript t7 transcription kit am1334
Megascript T7 Transcription Kit Am1334, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/megascript+t7+transcription+kit+am1334/megascript+t7+transcription+kit/pmc12271581-214-6-10
Average 90 stars, based on 1 article reviews

megascript t7 transcription kit am1334 - by Bioz Stars, 2026-09

90/100 stars

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Synthesized:

Article Title: A noncanonical role of roX RNAs in autosomal epigenetic repression
Article Snippet: .. RNA was synthesized by in vitro transcription from a T7-promoter containing PCR product (primers were listed in Supplementary Data ) using the MEGAscript T7 Transcription Kit (AM1334, Thermo Fisher), biotinylated using the Pierce RNA 3′ End Desthiobiotinylation Kit (20163, Thermo Fisher), and incubated with purified recombinant His-tagged protein from E.coli . ..

Article Title: MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control
Article Snippet: .. All mRNAs were synthesized using the MEGAScript T7 Transcription Kit (Thermo Fisher Scientific, no. AM1334) with 6 mM ATP, 6 mM CTP, 6 mM GTP, 6 mM N1-methylpseudo-UTP (m1Ψ) (TriLink BioTechnologies, no. N-1081) and 6 mM CleanCap Reagent AG (TriLink Biotechnologies, no. N-7113-10). .. Reaction mixtures were incubated at 37°C for 4 h and further incubated at 37°C for 30 min in the presence of TURBO DNase (Thermo Fisher Scientific) to remove the template DNA.

Article Title: A noncanonical role of roX RNAs in autosomal epigenetic repression.
Article Snippet: .. RNA was synthesized by in vitro transcription from a T7-promoter containing PCRproduct (primers were listed in Supplementary Data 6) using the MEGAscript T7 Transcription Kit (AM1334, Thermo Fisher), biotinylated using the Pierce RNA 3′ End Desthiobiotinylation Kit (20163, Thermo Fisher), and incubated with purified recombinant Histagged protein from E.coli. ..

In Vitro:

Article Title: Glucose binds and activates NSUN2 to promote translation and epidermal differentiation
Article Snippet: Full-length transcripts for GRHL1 and GRHL3 were cloned into linearized pGeneArt1 (pGA.1, Synthesized from Life Technologies, P01505112) vector. .. Approximately 1 μg linearized transcript-containing pGA.1 vectors were used as a template in an in vitro transcription reaction for 2 h at 37°C, using the MEGAscript T7 transcription kit (ThermoFisher, AM1334) following the manufacturer’s instruction. .. The resulting mixture was diluted with 50 μl RNase-free water, and the in vitro transcribed RNA was then purified using RNeasy Plus kit (QIAGEN, 74 136).

Article Title: Characterization of non-standard viral genomes during arenavirus infections identifies prominent S RNA intergenic region deletions.
Article Snippet: .. In vitro transcrip tion was performed with the Megascript T7 Transcription Kit (Thermofisher, AM1334), and RNA was isolated with LiCl precipitation according to the manufacturer’s protocols. .. Purified RNA was measured by Qubit and tested for quality by Bioanalyzer (Agilent).

Polymerase Chain Reaction:

Incubation:

Purification:

Recombinant:

Isolation:

Article Title: Characterization of non-standard viral genomes during arenavirus infections identifies prominent S RNA intergenic region deletions
Article Snippet: .. In vitro transcription was performed with the Megascript T7 Transcription Kit (Thermofisher, AM1334), and RNA was isolated with LiCl precipitation according to the manufacturer’s protocols. .. Purified RNA was measured by Qubit and tested for quality by Bioanalyzer (Agilent).

other:

Article Title: Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits
Article Snippet: Megascript T7 transcription kit , Thermofisher , AM1334.

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